A Label-Free and Ultrasensitive Immunosensor for Detection of Human Chorionic Gonadotrophin Based on Graphene FETs
نویسندگان
چکیده
We report on a label-free immunosensor based on graphene field effect transistors (G-FETs) for the ultrasensitive detection of Human Chorionic Gonadotrophin (hCG), as an indicator of pregnancy and related disorders, such as actopic pregnancy, choriocarcinoma and orchic teratoma. Pyrene based bioactive ester was non-covalently anchored onto the graphene channel in order to retain the sp² lattice. The G-FET transfer characteristics showed repeatable and reliable responses in all surface modifying steps using a direct current (DC) readout system. The hCG concentration gradient showed a detection limit of ~1 pg·mL-1. The proposed method facilitates the cost-effective and viable production of graphene point-of-care devices for clinical diagnosis.
منابع مشابه
The potentiality of the functionalized nitrogen and thiol-doped graphene quantum dots (GQDs-N-S) to stabilize the antibodies in the designing of human chorionic gonadotropin immunosensor
In this study, for the first time, a simple immunosensor for ultrasensitive recognition of Human Chorionic Gonadotropin (hCG) in serum samples was fabricated by exploiting a simple approach. In this method, a low-cost and sensitive immunosensor was fabricated based on QDs-N-S/Au nanoparticles (NPs) modified Screen-Printed Carbon Electrode (SPCE). It seems that, QDs-N-S/Au NPs/ antibody as a bio...
متن کاملFabrication of an Electrochemical Immunosensor for Determination of Human Chorionic Gonadotropin Based on PtNPs/Cysteamine/AgNPs as an Efficient Interface
An ultrasensitive electrochemical immunosensor for the detection of tumor marker human chorionic gonadotropin (hCG) was developed with a limit of detection as low as 2 pg mL-1 in phosphate buffer. The Platinum nanoparticles (PtNPs) were electrodeposited to modify the gold surface and to increase enlarging the electrochemically active sites, resulting in the facilitation of electron exchange. Cy...
متن کاملUsing Boehmite Nanoparticles as an Undercoat, and Riboflavin as a Redox Probe for Immunosensor Designing: Ultrasensitive Detection of Hepatitis C Virus Core Antigen
In this study a label-free electrochemical Immunosensor for ultrasensitive detection of Hepatitis C virus core antigen in serum samples was fabricated by using a simple approach. In this method a low-cost and sensitive immunosensor was fabricated based on a boehmite nanoparticles (BNPs) modified glassy carbon. The BNPs provide a specific platform with increased surface area which is capable of ...
متن کاملInvestigation of immunosensor modification with reduced Graphene Oxide with Au Nanoparticles on glassy carbon electrode in Label-free for Escherichia coli detection
Escherichia coli is an indicator in the quality control of pharmaceutical and other samples. Reduced graphene oxide (rGO) as a kind of carbon compositions was immobilized on glassy carbon electrode (GCE). Chronoamperometric and reduction methods were used for Au NPs decoration and it completed with polyclonal E. coli antibody and 0.5 W/V% Bovine Serum Albumin solution. Morphology and structure ...
متن کاملAn ultrasensitive label-free electrochemical immunosensor based on signal amplification strategy of multifunctional magnetic graphene loaded with cadmium ions
Herein, a novel and ultrasensitive label-free electrochemical immunosensor was proposed for quantitative detection of human Immunoglobulin G (IgG). The amino functionalized magnetic graphenes nanocomposites (NH2-GS-Fe3O4) were prepared to bond gold and silver core-shell nanoparticles (Au@Ag NPs) by constructing stable Au-N and Ag-N bond between Au@Ag NPs and -NH2. Subsequently, the Au@Ag/GS-Fe3...
متن کامل